重組抗體
- SARS-CoV-2 Spike RBD Nanobody
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恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗)，包括人類常用抗體，模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。
恒遠生物擁有專業的質控團隊，配備先進實驗儀器，現已成功地建立了ChIP平臺，因此我們的抗體不僅能提供Biotin、FITC、HRP等標記，大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。
我們致力于為所有用戶提供高親和力、高特異性的優質抗體，歡迎來電咨詢。
SARS-CoV-2 Spike RBD Nanobody
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貨號：HYK-200093
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規格：
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圖片：
產品詳情
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產品描述：
This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA).
It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/ml IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM).
Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins. -
Uniprot No.：P0DTC2
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別名：Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov S1 Antibody;Anti-ncov spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus S1 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-Spike RBD Antibody; E2 Antibody; E2 glycoprotein Antibody; Human coronavirus spike glycoprotein Antibody; S Antibody; SARS-CoV-2 S1 RBD Antibody; S glycoprotein Antibody; Spike glycoprotein Antibody
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反應種屬：Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
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免疫原：Recombinant Human Novel Coronavirus Spike glycoprotein(S) (319-541aa) (CSB-YP3324GMY1 and CSB-MP3324GMY1b1)
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免疫原種屬：Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：VHH fusion with human IgG1 Fc
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純化方式：Affinity-chromatography
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克隆號：A1
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, GICA, Neutralising
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推薦稀釋比：
Application Recommended Dilution ELISA 1:10000-1:100000 GICA 1:10000-1:40000 Neutralising 1:100-1:10000 -
Protocols：ELISA Protocol
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
N Antibody
SARS-CoV-2 Spike RBD Nanobody, HRP conjugated
SARS-CoV-2 Spike RBD Nanobody, FITC conjugated
SARS-CoV-2 Spike RBD Nanobody, Biotin conjugated
Phospho-Histone H3.3 (T3) Antibody
Di-methyl-Histone H3.1(K4)Antibody
在線詢價
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