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His-Tag Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

His-Tag Monoclonal Antibody

貨號(hào)：

HYK-200080
規(guī)格：
圖片：
IP antibody use: 5μg His Mouse IgG1 per ml Lysate, WB 1:3000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with His-tag fusion protein
Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
Lane 6: Recombinant protein (E.coli)

產(chǎn)品詳情

產(chǎn)品描述：

His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
宿主：

Mouse
反應(yīng)種屬：

N/A
免疫原：

Synthetic Peptide
標(biāo)記方式：

Non-conjugated
抗體亞型：

IgG
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.